Utility of other investigations for children and adolescents with musculoskeletal symptoms
Synovial fluid aspiration should only be performed in children or adolescents with suspected septic arthritis as part of acute hospital management. Local or general anaesthesia may be required, depending on the child or adolescent’s age and the affected joint. Detection of a pathogen on culture of synovial fluid is diagnostic of infection. Nucleic acid amplification testing (NAAT) (eg polymerase chain reaction [PCR]) of synovial fluid can identify microorganisms not detected by synovial fluid culture.
Synovial fluid aspiration for microscopy alone is not usually helpful in the differential diagnosis of inflammatory arthritis in children or adolescents. Crystal deposition disease (eg gout, calcium pyrophosphate deposition) is extremely rare in childhood.
Urinalysis is important to detect proteinuria or haematuria in children or adolescents with suspected kidney disease associated with immunoglobulin A (IgA) vasculitis or inflammatory connective tissue disease such as SLE; sterile pyuria may be present in reactive arthritis.